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Rossana Neffa de los Rios (born 16 September 1975) is a retired tennis player from Paraguay. She was born in the Paraguayan capital, Asunción, and now resides in Miami, Florida, with her daughter Ana Paula Neffa de los Ríos, who is also a professional tennis player, and her husband Gustavo Neffa, a retired Paraguayan Association football player who played for Boca Juniors. De los Ríos achieved a career-high WTA rankings of No. 51 in singles and 52 in doubles. She played doubles with Grand Slam champions such as Maria Sharapova, Svetlana Kuznetsova, Arantxa Sánchez Vicario, as well as Jelena Janković and Dinara Safina. She is also a former world number one junior player, having won the 1992 Roland Garros Juniors event. De los Ríos participated in six editions of the (1994, 2001, 2002, 2003, 2009 and 2010) of the Australian Open. She was the only female tennis representative for Paraguay in the 1992 and 2000 Olympic Games. De los Ríos was also in Paraguay's Fed Cup team in 1991 and 1992, and the Pan American team in 1999.